Journal: BMC Neuroscience

Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study

doi: 10.1186/s12868-025-00993-1

Figure Lengend Snippet: EV surface marker changes after RIC treatment: The expression of the EV-markers CD62 and MCP-1 is plotted showing the Log 2 fold changes compared to the baseline sample of each patient. The p-value is shown for both the significant changes within RIC group (red) and compared with the miRNA expression changes over time in Sham group (grey). ( a ) CD62 shows an upregulation in RIC treated patients compared to Sham. ( b ) MCP-1 showed no significant changes in between the different timepoints

Article Snippet: CD62 E/P , R & D Systems , BBA1 , BBIG-E(13D5).

Techniques: Marker, Expressing

Journal: BMC Neuroscience

Article Title: Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study

doi: 10.1186/s12868-025-00993-1

Figure Lengend Snippet: Correlation analysis of significant miRNA measurements (hsa-miR-19b-3p, hsa-miR-30d-5p, hsa-miR-374a-5p, hsa-miR-20a/20b-5p, hsa-miR-24-3p) and CD62/P-selectin. The histograms on the diagonal show the distribution of each variable. The lower triangular panels depict scatter plots with fitted lines, illustrating the linear relationships between pairs of variables. The upper triangular panels display the correlation coefficients, with significance levels indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001)

Article Snippet: CD62 E/P , R & D Systems , BBA1 , BBIG-E(13D5).

Techniques:

Journal: Stem Cells International

Article Title: Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells

doi: 10.1155/2016/1406304

Figure Lengend Snippet: Interaction of LO-EPC with activated HAEC. Surface expression of adhesion molecules in HAEC after stimulation with 0.05 ng/mL TNF α (a). Surface expressions of E-selectin, ICAM-1, and VCAM-1 were quantified using flow cytometry. The graph shows relative Mean Fluorescence Intensity (MFI) normalised to untreated cells and represents the mean ± SE of three experiments. ∗∗ p < 0.01. Activation of HAEC did not increase the interaction with LO-EPC (b). 4 × 10 5 LO-EPC were perfused to 3 × 10 4 HAEC for 4 min and the data represented as mean ± SE of three experiments.

Article Snippet: To study TNF α mediated HAEC activation, control HAEC and HAEC treated with 0.05 ng/ μ L TNF α (R&D System, UK), for 4 hours at 37°C, were collected in 100 μ L 1% bovine serum albumin (BSA, Sigma) in PBS and incubated with fluorescein conjugated anti-human E-selectin antibodies (R&D System), APC conjugated anti-human ICAM-1 (BD), and PE/Cy5 conjugated anti-human VCAM-1 (Bio Legend, UK), together with the respective isotype control antibodies.

Techniques: Expressing, Flow Cytometry, Fluorescence, Activation Assay

Journal: Frontiers in Medicine

Article Title: Efficacy evaluation and predictive value of IL-20 and Apelin-13 after cataract surgery by phacoemulsification combined with IOL implantation

doi: 10.3389/fmed.2025.1737194

Figure Lengend Snippet: ROC curve of serum IL-20 (A) , serum Apelin-13 (B) , aqueous humor IL-20 (C) , and aqueous humor Apelin-13 (D) levels for predicting poor efficacy of phacoemulsification combined with IOL implantation surgery in cataract patients.

Article Snippet: The human IL-20 ELISA kit (R&D Systems, USA; Catalog # DY724) from lot # 2365891 (expiry: 03/2025) and the human Apelin-13 ELISA kit (Phoenix Pharmaceuticals, USA; Catalog # EK-057-23) from lot # PHX1022 (expiry: 02/2025) were used.

Techniques:

Journal: Frontiers in Medicine

Article Title: Efficacy evaluation and predictive value of IL-20 and Apelin-13 after cataract surgery by phacoemulsification combined with IOL implantation

doi: 10.3389/fmed.2025.1737194

Figure Lengend Snippet: ROC curve of serum IL-20/Apelin-13 ratio (A) and aqueous humor IL-20/Apelin-13 ratio (B) for predicting poor efficacy of phacoemulsification combined with IOL implantation surgery in cataract patients.

Article Snippet: The human IL-20 ELISA kit (R&D Systems, USA; Catalog # DY724) from lot # 2365891 (expiry: 03/2025) and the human Apelin-13 ELISA kit (Phoenix Pharmaceuticals, USA; Catalog # EK-057-23) from lot # PHX1022 (expiry: 02/2025) were used.

Techniques:

Journal: Cancers

Article Title: Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma

doi: 10.3390/cancers11111672

Figure Lengend Snippet: ( A ) Cell flow analysis of human head and neck squamous cell carcinoma cell lines (HNSCC) cells on rhE-selectin-Fc-chimera and on confluent monolayers of IL-1α-stimulated and unstimulated human umbilical vascular endothelial cells. UTSCC 24B cells most strongly adhered to rhE-sel, and UTSCC 24A cells showed the highest number of adhesive events for stimulated HUVECs. Incubation with the adhesion blocking anti-E-selectin mAb significantly reduced the tethering of UTSCC 24A and tethering and rolling of UTSCC 24B cells to HUVECs (* p < 0.05, ** p < 0.01, *** p < 0.001). ( B ) Representative flow cytometric histograms of selectin binding and canonical selectin ligand expression. All HNSCC cells bound to rhP-selectin, but only UTSCC 24A and B cells bound to rhE-selectin. HNSCC cells did not express CA19-9 (sLeA), but 23% of UTSCC 24A and 29% of UTSCC 24B cells expressed CD15s (sLeX).

Article Snippet: E- and P-selectin (724-ES, 137-PS, rhE- and rhP-selectin/IgG-Fc chimera, R&D Systems, Minneapolis, Minnesota, USA) binding sites were analyzed with and without pronase pretreatment.

Techniques: Adhesive, Incubation, Blocking Assay, Binding Assay, Expressing

Journal: Cancers

Article Title: Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma

doi: 10.3390/cancers11111672

Figure Lengend Snippet: ( A ) Binding of UTSCC 24A and 24B cells to HUVECs with and without pre-incubation with the E-sel antibody was not significantly influenced by proteolytic pretreatment of tumor cells with pronase. ( B ) Pronase treatment slightly reduced static E-selectin binding and CD15s (sLeX) expression in UTSCC 24A, but not in UTSCC 24B cells.

Article Snippet: E- and P-selectin (724-ES, 137-PS, rhE- and rhP-selectin/IgG-Fc chimera, R&D Systems, Minneapolis, Minnesota, USA) binding sites were analyzed with and without pronase pretreatment.

Techniques: Binding Assay, Incubation, Expressing